TABLE 3.

Toxocara sp. and Echinococcus multilocularis primers and hydrolysis probes for qPCR, designed for a partial sequence of the cox1 gene of Toxocara cati and for the rrnL gene of E. multilocularis

Host species	Primer or probe	Oligonucleotide sequence (5′–3′)	Reference
Toxocara spp.	Tox forward primer	AAAATAGCCAAATCCACACTACTACCA	This study
	Tox reverse primer	GGTGTGGGACTAGTTGAACTGTGTA	This study
	Tox probe	FAM-CCCCATAGTCCTCAAAG-MGB	This study
	Tox forward primer (SEQ-PCR)	TGGATGTTACCTTTGATGTTGGG	This study
E. multilocularis	rrn-Em forward primer	CTGTGATCTTGGTGTAGTAGTTGAGATTT	23
	rrn-Em reverse primer	GGCTTACGCCGGTCTTAACTC	23
	rrn-Em probe	FAM-TGGTCTGTTCGACCTTTTTAGCCTCCAT-TAMRA	23
	rrn-Em reverse primer (SEQ-PCR)	GGGGTCAATCACAACAACCC	This study