TABLE 5.
Average Cq values for qPCRs performed 5 times independently on each point of the DNA ranges for the 5 carnivores studieda
| Host species | Mean (SD) Cq at indicated DNA concn (ng/μl) | ||
|---|---|---|---|
| 50 ng/µl | 5 ng/µl | 0.5 ng/µl | |
| Vulpes vulpes | 16.9 (1.41) | 20.4 (1.01) | 24.5 (1.46) |
| Canis lupus familiaris | 13.9 (1.02) | 17.6 (1.85) | 21.0 (0.73) |
| Felis silvestris catus | 15.8 (0.78) | 20.1 (0.33) | 24.0 (0.77) |
| 40 ng/µl | 4 ng/µl | 0.4 ng/µl | |
| Martes foina | 12.4 (2.47) | 16.3 (2.69) | 21.4 (3.53) |
| 8 ng/µl | 0.8 ng/µl | 0.08 ng/µl | |
| Meles meles | 12.4 (0.43) | 16.2 (0.45) | 21.0 (0.55) |
a
All qPCRs were performed in duplicate.