FIG 1.

Sytox green uptake by yeast cells exposed to straight-chain alcohols and amphotericin B (AmB). The exponentially growing wild-type cells (BY 4742) were incubated in YPD medium containing the indicated concentrations of methanol (△), ethanol (□), 1-propanol (▲), or 1-butanol (■) (A) or of AmB (●) (B) at 30°C for 1 h. The cells with an OD₆₀₀ of 1 loaded with Sytox green were immediately used for fluorescence measurement at an excitation wavelength of 544 nm and an emission wavelength of 488 nm. The fluorescence intensities of the treated cells were normalized to that of the untreated control and plotted as relative fluorescence units (RFU). All experiments were carried out in triplicate. The error bars indicate the standard deviations (SD). v/v, vol/vol.