FIG 3.
Changes in cytosolic, vacuolar, and external pH in response to straight-chain alcohols and AmB in the wild-type strain. Cytosolic, vacuolar, and external pH values of the wild-type cells (BY4742) incubated in YPD medium containing 0 to 10% methanol (△), ethanol (□), 1-propanol (▲), or 1-butanol (■) (A to C) or 0 to 1 μM AmB (D) at 30°C for 1 h were measured. The log-phase wild-type cells transformed with pYES-PACT1-pHluorin were used for the measurement of cytosolic pH (A), whereas log-phase wild-type cells without the plasmid were used for the measurement of vacuolar pH (B) and external pH (C). All experiments were carried out in triplicate. The error bars indicate the SD.