TABLE 1.
Results of nominal logistic regression assessing the importance of cultivation variables to successful in vitro reintroduction of an endohyphal bacterium (Luteibacter sp. 9143) into living mycelium of Pestalotiopsis sp. 9143 (a foliar fungus; Sordariomycetes, Ascomycota)a
| Factor | n | df | χ2 | P |
|---|---|---|---|---|
| Age of bacterial culture | 1 | 1 | 2.44 | 0.1186 |
| MgCl2 wash | 1 | 1 | 47.38 | <0.0001 |
| Concn of PDB | 4 | 4 | 25.25 | <0.0001 |
| Mycelium/bacterium ratio | 2 | 2 | 3.72 | 0.1559 |
| Medium | 1 | 1 | 38.22 | <0.0001 |
Success of reintroduction (yes or no) was used as the response variable. The explanatory variables are shown in all the other columns in Table S1 in the supplemental material (age of the bacterial culture, whether cultures were washed with MgCl2, the concentration of PDB, the mycelium/bacterium ratio, and the medium on which cocultures were plated). The whole model was significant (chi-square = 83.87; df = 9; P < 0.0001), and the results of effect tests, determined by likelihood ratios, are shown here. The results of this analysis were used to prune the total data set to identify the factors associated with positive reintroduction, which are shown in Table 2.