TABLE 2.

In vitro reintroduction of Luteibacter sp. 9143 into Pestalotiopsis sp. 9143 always failed when axenic cultures were not washed with MgCl₂ and when cocultures were cultivated in 0.0001× PDB and failed in 29 of 30 trials when the cocultures were plated on 1× PDA^a

Coculture medium	Mycelium/bacterium ratio	Resynthesis
		1 day old	3 days old
1× PDB	5:1	No	Yes
	7:1	No	Yes
	10:1	Yes	No
0.1× PDB	5:1	No	Yes
	7:1	Yes	Yes
	10:1	Yes	No
0.01× PDB	5:1	Yes	Yes
	7:1	Yes	Yes
	10:1	Yes	Yes
0.001× PDB	5:1	No	Yes
	7:1	Yes	Yes
	10:1	Yes	Yes

^aShown are qualitative outcomes of resynthesis attempts when cultures were washed with MgCl₂, PDB concentrations were >0.0001×, and cocultures were plated on water agar for resynthesis attempts started with 1- and 3-day-old bacterial cultures. Nominal regression of this reduced data set did not reveal significant differences among the suites of treatments listed here; however, we note that resynthesis was always successful when 0. 0.001× PDB was used as the coculture medium, whereas success was more variable on other concentrations of PDB (see Table S1 in the supplemental material).