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Unwinding activity of Tk-EshA for DNA/RNA hybrid duplexes. Native PAGE demonstration used in the detection of helicase activity. Tk-EshA was added to assay mixtures containing 2 pmol of the RNA/DNA hybrid composed of oligonucleotides, RNA54 labeled at the 5′ terminus with Cy5.5, and DNA70. The amount of Tk-EshA was 0, 5, 10, 20, 40, or 60 pmol. Gray line, RNA; black line, DNA; *, fluorescent label; S, ssRNA; D, RNA/DNA hybrid substrate; T, trapped RNA/DNA hybrid.
