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Effect of Tk-EshA addition on PCR with a high-GC-content template. The toxA region (1,917 bp) with a high GC content (69%) from the Pseudomonas aeruginosa genome was amplified in the presence or absence of Tk-EshA. The annealing temperature in the PCR was 62°C, 65°C, or 68°C. The concentration of Tk-EshA was 0, 50, or 100 nM. DNA size markers are shown in lane M. The target DNA (1,917 bp) amplified by primers toxA Fw and toxA Rv is indicated by a black arrowhead.
