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Effect of Tk-EshA addition on PCR by family A DNA polymerases. 16S rRNA genes (1,498 bp) from the T. kodakarensis genome were targeted in PCR. 16S rRNA genes were amplified in the presence or absence of Tk-EshA. The concentration of Tk-EshA was 0, 2, 10, 50, or 100 nM. Lane M shows the 100-bp DNA ladder. PCR results using a DNA polymerase from T. aquaticus (A) or from T. thermophilus (B) are shown.
