TABLE 1.
Strains and primers used in this study
| Strain or primer | Relevant characteristic(s) or sequence (5′ to 3′)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | Stratagene |
| BL21-CodonPlus(DE3)-RIL | E. coli B F− ompT hsdS(rB− mB−) dcm+ Tetr gal λ(DE3) endA Hte [argU ileY leuW Camr] | Stratagene |
| Primers | ||
| tk0566-Fw | AAAAAACATATGCTCTTCGTCGTGAGACCGGGA | This study |
| tk0566-Rv | TTTGAATTCTTAGCGTATCATCCTCCCTTCTTCTA | This study |
| tk0928-Fw | AAAAAAACATATGATGGTTGTCCTGAGAATCCC | This study |
| tk0928-Rv | AAAAGTCGACTTAACTAGAGCGGCGCTTTTTGG | This study |
| TK0566-D344A-E345A Fw | GGAACCATAGTGATAGCCGCCATACACACGCTCGAC | This study |
| TK0566-D344A-E345A Rv | GTCGAGCGTGTGTATGGCGGCTATCACTATGGTTCC | This study |
| 16S rRNA gene Fw | ATTCCGGTTGATCCTGCCGGAGGCCACTGC | This study |
| 16S rRNA gene Rv | TCCGGCGATAGGAGGTGATCGAGCCGTAGG | This study |
| toxA Fw | ATGCACCTGACACCCCATTG | This study |
| toxA Rv | TTACTTCAGGTCCTCGCGCG | This study |
| 16 S miss 5′ Rv | ATGGAGGCGAGCTCGAAGCTCGCCCGACAC | This study |
| 16 S miss 3′ Rv | GGCGAGCTCGAAGCTCGCCCGACACAGCTA | This study |
a
Mismatched nucleotides within the sequences of the 16S rRNA, Tk0566, or Tk0928 gene are underlined.