FIG 2.

Detection of HBGA-like carbohydrates on lettuce leaves with anti-HBGA monoclonal antibodies. Paraffin slides of lettuce were digested with 1 μg/μl cellulase R-10 at room temperature (RT) for 1 h before addition of PBS (no anti-HBGA), anti-A-type HBGA monoclonal antibody (A type), anti-B-type HBGA monoclonal antibody (B type), or anti-H-type HBGA monoclonal antibody (H type). Porcine intestine paraffin slides were used as positive controls for A-type and H-type antigens and were digested with proteinase K at RT for 10 min before incubation with antibodies. The binding signal (green) was detected by Alex Fluor 488-conjugated goat anti-mouse IgG antibody. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). All pictures were taken using the same exposure time.