Fig. 3.
Cyclase assay: (A) 2AG (▪) (EC50 4.1 nM) and the stable analog of anandamide, methanandamide (▲) (EC50 20 nM), were intermediate efficacy agonists of the cyclase pathway, with 2AG being more potent. CP55940 (●) inhibition of adenylyl cyclase is shown for reference. (B) AM630 (▪), JTE907 (▲), and SR144528 (▼) behaved as inverse agonists by increasing the accumulation of cAMP above that seen with forskolin alone. EC50 and Emax were obtained by fitting the dose-response curve using nonlinear regression with GraphPad Prism 4.0. Data represent mean ± S.E.M. of at least three experiments.