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Concentration-response curves for mCB1-mediated arrestin recruitment by CP55940 (●), 2AG (▪), and the stable anandamide analog methanandamide (▲). 2AG and methanandamide were low efficacy agonists in arrestin recruitment to CB1 receptor. EC50 and Emax values were obtained by fitting the dose-response curve using nonlinear regression with GraphPad Prism 4.0. Data represent mean ± S.E.M. of at least three experiments.
