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. 2016 May 31;82(12):3481–3492. doi: 10.1128/AEM.00371-16

FIG 3.

FIG 3

Development of an efficient conjugation protocol for the rapamycin producer S. iranensis HM 35. Stepwise modification of the basic protocol (23) led to increased conjugation efficiency and allowed reproducible generation of gene deletion mutants of S. iranensis HM 35. The highest conjugation efficiency (6.8 × 10−7 cells ml−1) was achieved when all modifications were applied in a single experiment.