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Identification of rapamycin by LC-mass spectrometry. Extracted ion chromatogram (EIC) showing mass-to-charge ratios (m/z) of 912 to 914 [M-H], corresponding to rapamycin extracted from biomass. Unlike with the ΔrapK mutants obtained by each modification of the transformation protocol, a mass corresponding to rapamycin was detectable only in S. rapamycinicus ATCC 29253 and S. iranensis HM 35.
