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Growth of S. iranensis HM 35 and ΔhutF mutants on ISP9 agar plates. Plates were inoculated with 5 × 105 spores and incubated for 14 days at 28°C. (1) Inoculation scheme; (2) plate supplemented with 1% (wt/vol) sucrose and 1% (wt/vol) ammonium sulfate (control); (3) plate supplemented with 1% (wt/vol) sucrose and 25 mM histidine. Growth on all other agar plates containing histidine as the sole C or N source was comparable to that on plate 3.
