FIG 2.

Copy numbers of cellodextrin transporter gene (cdt-1) and β-glucosidase gene (gh1-1) in the parental (EJ1) and evolved (EJ2) strains. (A) Copy numbers of cdt-1 and gh1-1 estimated by genome sequencing data and qPCR. (B) The β-glucosidase activity of engineered S. cerevisiae EJ1 and evolved strain EJ2. The results are the means from triplicate experiments, and the error bars indicate standard deviations. Cells grown to mid-log phase in YP medium containing cellobiose (20 g/liter) were harvested for the enzymatic assay.