TABLE 2.
Changes in cellobiose fermentation parameters after serial subculturesa
| Parameter (unit) | Value (mean ± SD) |
||
|---|---|---|---|
| EJ1 | EJ2 | EJ2-10 | |
| Cellobiose consumption rate (g/[liter · h]) | 0.18 ± 0.00 | 2.73 ± 0.01 | 2.62 ± 0.10 |
| Specific cellobiose consumption rate (g/[g cell · h]) | 0.09 ± 0.00 | 0.49 ± 0.00 | 0.46 ± 0.01 |
| Ethanol yield (g/g cellobiose) | 0.40 ± 0.00 | 0.40 ± 0.00 | |
| Volumetric ethanol productivity (g/[liter · h]) | 1.09 ± 0.00 | 1.04 ± 0.03 | |
| Specific ethanol productivity (g/[g cell · h]) | 0.19 ± 0.00 | 0.18 ± 0.00 | |
a
Fermentation experiments were performed under oxygen-limited conditions (100 rpm) in YP medium with 80 g/liter of cellobiose, and the initial cell density was adjusted to 0.29 g/liter (OD600 = 1). The parameters were measured at 29 h. The results are the means from duplicate experiments. Serial subcultures were performed under oxygen-limited conditions (100 rpm) in YP medium with 80 g/liter of cellobiose or 20 g/liter of glucose, and the initial cell density of each subculture was adjusted to 0.29 g/liter (OD600 = 1).