EDTA-sensitive phenotypes of strains containing double mutations of genes encoding zinc uptake proteins and zinc chaperones. The EDTA sensitivities of TCZA15 (troC::Gm znuA::Km), TCZT14 (troC::Gm zinT::Km), TCYC15 (troC::Gm yciC::Km), and ZTYC15 (zinT::Gm yciC::Km) were compared to that of wild-type NTL4 (WT) and TC142 (troC::Gm). Sensitivity to EDTA on plates containing AB and AB plus EDTA (0.3, 0.6, 0.9, and 1.2 mM) with or without 50 μM ZnCl2 was assessed as described for Fig. 3.