TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Agrobacterium tumefaciens | ||
| NTL4 | WT strain: a Ti plasmid-cured derivative of strain C58 | 37 |
| PC135 | zinT::pKNOCK-Gm (zinT::Gm); Gmr | 25 |
| PS132 | znuA::pKNOCK-Gm (znuA::Gm); Gmr | 25 |
| SPP12 | zur::pKNOCK-Gm (zur::Gm); Gmr | 25 |
| TC142 | troC::pKNOCK-Gm (troC::Gm); Gmr | This study |
| TCZA15 | troC::pKNOCK-Gm and znuA::pKNOCK-Km (troC::Gm znuA::Km); Gmr and Kmr | This study |
| TCZT14 | troC::pKNOCK-Gm and zinT::pKNOCK-Km (troC::Gm zinT::Km); Gmr Kmr | This study |
| TCYC15 | troC::pKNOCK-Gm and yciC::pKNOCK-Km (troC::Gm yciC::Km); Gmr Kmr | This study |
| YC154 | yciC::pKNOCK-Km (yciC::Km); Kmr | This study |
| ZAYC15 | znuA::pKNOCK-Gm and yciC::pKNOCK-Km (znuA::Gm yciC::Km); Gmr Kmr | This study |
| ZTYC15 | zinT::pKNOCK-Gm and yciC::pKNOCK-Km (zinT::Gm yciC::Km); Gmr Kmr | This study |
| ZURYC15 | zur::pKNOCK-Gm and yciC::pKNOCK-Km (zur::Gm yciC::Km); Gmr Kmr | This study |
| Escherichia coli | ||
| DH5α | Host for general DNA cloning | 38 |
| BW20767 | Host for plasmid pKNOCK-Gm and pKNOCK-Km | 39 |
| Plasmids for gene inactivation | ||
| pKNOCK-Gm | Suicide vector; Gmr | 40 |
| pKNOCK-Km | Suicide vector; Kmr | 40 |
| pKNOCKTROC | Internal coding region of troC cloned into pKNOCK-Gm; Gmr | This study |
| pKNOCKmYCIC | Internal coding region of yciC cloned into pKNOCK-Km; Kmr | This study |
| pKNOCKmZINT | Internal coding region of zinT cloned into pKNOCK-Km; Kmr | This study |
| pKNOCKmZNUA | Internal coding region of znuA cloned into pKNOCK-Km; Kmr | 25 |
| Plasmids for complementation | ||
| pBBR1MCS-4 | Expression vector; Apr (pBBR) | 41 |
| pTROC | Full-length troB cloned into pBBR1MCS-4; Apr | This study |
| pTROCBA | Full-length troCBA operon cloned into pBBR1MCS-4; Apr | This study |
| pYCIC | Full-length yciC cloned into pBBR1MCS-4; Apr | This study |
| pZINT | Full-length zinT cloned into pBBR1MCS-4; Apr | 25 |
| pZNUA | Full-length znuA cloned into pBBR1MCS-4; Apr | 25 |
| pZUR | Full-length zur cloned into pBBR1MCS-4; Apr | 25 |
| Promoter-lacZ transcriptional fusions | ||
| pUFR027lacZ | Promoter probe vector; Tcr | 42 |
| p027troC-lacZ | 266-bp DNA fragment containing troC promoter fused to lacZ of pUFR027lacZ | This study |
| p027yciC-lacZ | 274-bp DNA fragment containing yciC promoter fused to lacZ of pUFR027lacZ | This study |
| YciC-LacZ and YciC-PhoA translational fusions | ||
| pPR9TT | Reporter vector, LacZ as a reporter; Apr | 43 |
| pYCLacZ | YciC amino acid residues 1–400 fused to LacZ of pPR9TT; Apr | This study |
| p'PhoA | 5′-truncated phoA gene lacking its signal peptide sequence cloned into pBBR; Apr | 44 |
| pYCPhoA | YciC amino acid residues 1–400 fused to PhoA of p′PhoA; Apr | This study |
| Plasmid for tumor assay | ||
| pCMA1 | pTiC58traM::nptII; Kmr | 45 |
a
Apr, ampicillin resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.