TABLE 1.
Primer sets used in this study
| Analysis and region | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| RT-qPCR analyses | ||
| atu0043 | GCATATCGATGACCACCACA | TTGACGTTCCACACAAGGAA |
| atu0140 | ATGAGCGACTGCCTGATCTT | GCCATAGGTGATGGAGGAGA |
| atu0161 | AAACGGTTCAGCCTTCACAG | TCGGCGTTAACGGTTTCTAC |
| atu0229 | GGTTTGGCAAGATCGTCAAT | CGATGCCGTGAAAATAGACC |
| atu0231 | GCGAACAGAATGCGGTAGAT | AAGATCGGGAAGTTCTGCTG |
| atu0786 | GTTGCCGGTTACGTCTTCAT | TTGTTTTCCCAGACGAGCTT |
| atu1028 | CAAGCTTCTGAAGGCGTTTC | TCCATGTTGCCCTTGATCTT |
| atu1226 | ACGACGCTTGAAAAGCTGAT | CCAGGCTGACAAGGATGATT |
| atu1331 | GTCGGCACGGTCTATCTCTC | GACGATGATTTCCACCACCT |
| atu1413 | GCGAAAAGCTGAAGGTCATC | GGCATGACGAAGTTCCATTT |
| atu1414 | GAGCTTCTGAAGGCAACGTC | GAAAAGCATCGGCAAAACC |
| atu1415 | GGCCTTCGTATTCTCGTCAA | TTGTCTTGGCGACTGTCTTG |
| atu1416 | CCCTATCCGCAGAAATTGAA | ACCGAAAGGTCATGATCGAG |
| atu1417 | CGACATGATGCTGACTGGAC | ACGGCATAGTTGGACAAAGG |
| atu1418 | ATGGGTTGGTCGAGTTTTTG | GGATACCATGTCGGCTATCG |
| atu1419 | GGTGATCGAACTCAGGGAAA | TACTTCAATGGCATCCACGA |
| atu1420 | GCCTATGGCAAGTGGAATGT | CGAGCCGATATTGATCCAGT |
| atu1426 | CGTATGGGTTCGGAAGTGTT | AGCCTTTTCGATCGACTTCA |
| atu1507 | CAGGATGTGGAAAGCCTGAT | TTGACGATCTGCTGGATACG |
| atu1823 | CAAGTCGACGCTGTCCTACA | AACCTTCAGGAACTGCATGG |
| atu1870 | TCATCTGGCAGCTCATCAAG | GGCTCTTCCACATCTGCTTC |
| atu1923 | GAGCGTGGTTTCGGTCTTAC | CATCTTGATGGCGATTTCCT |
| atu1924 | CGACCTTCAACAACACGATG | CGTGTTCCTGAGCCTTCTTC |
| atu1998 | GACACCGTCTTCAACAAGCA | CACCCTCTTTATCCGTGGAA |
| atu2073 | CTGACGATCGAGATGACGAA | TCGACGGATACGAAATAGGC |
| atu2085 | GGACTGCTCGGATTTCAGAC | ACTGACTGGGATAGCGAACG |
| atu2449 | CGGCCAGTGTGTTTCAACTA | AAGACTTACGGATGGCGTTG |
| atu2497 | ACCTATGATCATCGCGGAAC | CAGGCATTGATGAGCACAAG |
| atu2623 | CTGATTTCCGAAGGCAAGAC | GAGCGAGATCACCTTCTTGG |
| atu2829 | ATTTGCTCGGTTTCGAGATG | ACCTTCCAGGGCAAAGAACT |
| atu3327 | TCGGCTGAACAGACACTCAG | TCGGAGAGTTTCACCAGGAC |
| atu3391 | GCCAAGGTCACCGATACACT | GGTTGGAAGCGATGATCTGT |
| atu3564 | GGTGCAGACACCTTCGTTTT | ACGATCTGTCGAGGCTGAGT |
| atu3677 | GTCCTGACGAAAGCAGTTCC | CTTCGCAACTCATGCAGAAA |
| atu3687 | GACGTCCATAACGTCGGACT | GAGGCGATCCATTCCTTGTA |
| atu3916 | ACTGGTCGCTTTCGCATACT | CGAGAACGGTGTTACGGATT |
| atu3918 | GAACTGGGTCTCGTCTTTGC | GCCAGAACAGGCTTGATAGG |
| atu4177 | CTCGATGAATATCGCAAGCA | ATCAGGTCGATTGCCTTGTC |
| atu4333 | GTCGAGATCACCGAGGAAAG | CAGATGTGCGAGGTTGAGAA |
| atu4544 | GTAAACCACCGGATCGATTG | GGTGGTTGGCGTAGATGAGT |
| atu4547 | AGGGACCGAAGAACCTGACT | GGCCAGATACCGGTCTGTAA |
| atu4709 | GGATGAAAAGCTGGTGAGGA | AAACACAAGCGGCAGAAACT |
| atu5311 | GCTGAAACGATCAAGGAAGC | CGCCGATATAGGGCAGATAA |
| atu5449 | TGACTATGCACCGCTGTTTC | ACGGTCAGAAGGTTGGATTG |
| atu6151 | CAGTGATGCGAACGTTTCTG | TTAGCCATGGCCATTCTTTC |
| Inactivation of SpG8-3 gene cluster | ||
| Region upstream of atu3663 | CCGGTTCTACATCCTGGAAA | CCTGCTCAACAGGCTACTCC |
| Region downstream of atu3693 | GACAACATGCCCCTCTCCTA | TCTGGAACGTCACCGACATA |
| nptII gene | TTGCTGCGCGCGACATCAAGGTTTCGACCGAGGAGTAGCCTGTTGAGCAGGTGTGTAGGCTGGAGCTGCTTC | TGAAAATGCCGCTGTATTTCCTCGATCACGTAGGAGAGGGGCATGTTGTCCATATGAATATCCTCCTTA |
| Region for inactivation diagnosis | GAGAGTGACGCTTTGGCTCT | GGTTGATCTGGTGCAGCTTT |