TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference(s) |
|---|---|---|
| Streptomyces strains | ||
| S. luteocolor NBRC 13826 | BD-12 producer | 14, 15 |
| S. lividans TK23 | Host strain for gene expression experiments | 25 |
| S. avermitilis SUKA17 | Host strain for gene expression experiments | 22 |
| Plasmids | ||
| pKU493A_aac(3)IV | Apmr, oriT of RK2, pMB1 ori; ϕK38-1-integrating vector derived from pKU460 | 22 |
| pKU1016 | Neor (aphII), oriT of RK2, pMB1 ori; ϕC31-integrating vector derived from pKU460 | 22 |
| pKU518 | Ampr Neor (aphII); ϕBT1-integrating BAC vector | 24 |
| pRED-sacB-aph | Neor (aphII) sacB Cmr; cloning vector for E. coli | 22 |
| pKU518BD12P2-C23 | pKU518 carrying the genomic DNA fragment (200,999 bp) that contains the BD-12-biosynthetic gene cluster (34 kbp) from S. luteocolor NBRC 13826 | This study |
| pRED/BD-12cluster | pRED-sacB-aph carrying the BD-12-biosynthetic gene cluster (34 kbp) from S. luteocolor NBRC 13826 | This study |
| pKU493A-BD12 | pKU493A_aac(3)IV carrying the BD-12-biosynthetic gene cluster (34 kbp) from S. luteocolor NBRC 13826 | This study |
| pKU493A-STΔorf18 | pKU493A_aac(3)IV carrying the ST-biosynthetic gene cluster (30 kbp) with in-frame deletion of the orf18 gene (STΔorf18) from S. rochei NBRC12908 | This study |
| pKU1016-orf11 | pKU1016 carrying the orf11 gene from the BD-12-biosynthetic gene cluster | This study |
a
Apmr, apramycin resistant; Neor, neomycin resistant; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant.