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. 2016 Jun 30;82(14):4190–4199. doi: 10.1128/AEM.00599-16

FIG 4.

FIG 4

Detection of nirS and nirK genes in 18PW and pure cultures of tNRB. (A) Ratio of nirS/16S rRNA gene copies in incubations with VFA and nitrate at the indicated temperatures of 18PW. (B) Ratio of nirS/16S rRNA gene copies in incubations with VFA and nitrate of isolates Thauera sp. TK001, Petrobacter sp. TK002, and Geobacillus sp. TK003 obtained in this study. (C) Detection of nirS genes by PCR with primers NirS2F and NirS3R in incubations of 18PW at 40 to 70°C, as indicated; qPCR detection of nirS in these incubations is shown in panel A. (D) Detection of nirK genes by PCR with primers GnirK2F and GnirK2R in incubations of 18PW as for panel C. (E) Detection of nirS genes by PCR with primers NirS2F and NirS3R in the following pure cultures: 1, Geobacillus thermoleovorans DSM5366; 2, G. kaustophilus DSM7263; 3, G. stearothermophilus DSM22; 4, G. thermodenitrificans DSM465; 5, G. toebii DSM14590; 6, G. thermocatenulatus DSM730; 7, G. subterraneus DSM13552; 8, G. galactosidasius DSM18751; 9, Tepidiphilus succinatimandens DSM15512; 10, Ammonifex degensii DSM10501; 11, Petrobacter (Tepidiphilus) sp. strain TK002; 12, Geobacillus sp. strain TK003; and 13, Thauera sp. TK001. (F) Detection of nirK genes by PCR with primers GnirK2F and GnirK2R in pure cultures as for panel E.