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TLC (A) and HPLC (B) analyses of Gly5M enzymatic reaction products using laminarin as the substrate. The reactions were performed at 30°C in 20 mM Tris-HCl (pH 6.0). Lane 1, glucose; lane 2, laminarin; lane 3, laminarin plus Gly5M for 5 min; lane 4, laminarin plus Gly5M for 30 min; lane 5, laminarin plus Gly5M for 60 min; lane 6, laminarin plus Gly5M for 120 min. The control contained substrate but lacked enzyme, and no incubation was performed.
