TABLE 1.
Cell experimental protocol
Group | Treatment on the indicated period bya: |
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---|---|---|---|
Intervention assay (0–24 h) | Therapy assay |
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0–24 h | 24–48 h | ||
Control | Medium | Medium | Medium |
Cd only | Medium + Cd | Medium + Cd | Medium |
8610 only | Medium + CCFM8610 | Medium | Medium + CCFM8610 |
Cd + 8610 | Medium + Cd + CCFM8610 | Medium + Cd | Medium + CCFM8610 |
Medium, RPMI 1640 medium supplemented with fetal bovine serum and penicillin-streptomycin; medium + Cd, 50 μM CdCl2 in medium; medium + CCFM8610, 1 × 108 CFU/well L. plantarum CCFM8610 in medium (without antibiotics); medium + Cd + CCFM8610, 50 μM CdCl2 and 1 × 108 CFU/well L. plantarum CCFM8610 in medium (without antibiotics). For the therapy assay, the cells were washed three times with phosphate-buffered saline after 24 h.