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Primer sequences used for real-time quantitative PCR
| Primera | Sequence (5′–3′) |
|---|---|
| β-actin-F | CCTAAGAGGAGGATGGTCGC |
| β-actin-R | CTCAACACCTCAACCCCCTC |
| ZO-1-F | TCTTCCATCATTTCGCTGTGT |
| ZO-1-R | TCTGAAACCATCAAGTCCACA |
| ZO-2-F | TCTCCGGACAATAGGACAACC |
| ZO-2-R | GTGGTTCCAGACAACGGACA |
| Occludin-F | TCACTTTTCCTGCGGTGACT |
| Occludin-R | GGGAACGTGGCCGATATAATG |
| Claudin-1-F | ATGCAAAGATGTTTTGCCACAG |
| Claudin-1-R | TACAAATTCCCATTGCAGCCC |
F, forward primer; R, reverse primer.
