. 2016 Jun 30;82(14):4253–4263. doi: 10.1128/AEM.00739-16

TABLE 3.

Specific activities of enzymes involved in 2NBA and 4NBA degradation by Cupriavidus sp. strain ST-14

Substrate	Activity (U/mg protein)^a
	2NBA nitroreductase in the presence of:		4NBA nitroreductase in the presence of:		3HAA dioxygenase	PCA dioxygenase	Ammonia-releasing enzyme
	NADPH	NADH	NADPH	NADH	3HAA dioxygenase	PCA dioxygenase	Ammonia-releasing enzyme
2NBA	0.128	0.012	<0.001	<0.001	0.046	<0.001	0.26
4NBA	<0.001	<0.001	0.244	0.021	<0.001	0.284	0.22
Succinate	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

For determination of specific activities, cell extracts were used as the sources of enzymes except for ammonia-releasing activity, where whole cells were used, and specific activities were measured from the results of transformation of MNBAs in resting-cell suspensions.