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Effect of Mn2+ on Michaelis-Menten saturation curves of direct oxidation (OII or RB5) by VP1. Data represent oxidation of OII in the absence (A) and presence (B) of 0.05 μM Mn2+ and oxidation of RB5 in the absence (C) and presence (D) of 0.05 μM Mn2+. Each reaction was repeated three independent times.
