TABLE 1.

Purification process for P. ostreatus VP1^a

Purification step	Vol (ml)	Protein concn (mg/ml)	Total protein (mg)	Activityb (U/ml)		Total activity (U)		Sp act (U/mg)		Yield (%)		Purification (fold)
				Direct	Mn2+ mediated	Direct	Mn2+ mediated	Direct	Mn2+ mediated	Direct	Mn2+ mediated	Direct	Mn2+ mediated
Crude	1,340.00	0.07	93.80	0.13	0.76	177.85	1,018.40	1.90	10.86	100.00	100.00	1.00	1.00
(NH4)2SO4c	200.00	0.14	28.80	0.65	3.50	130.00	700.00	4.51	24.31	73.09	68.74	2.38	2.24
HiTrap Q	19.80	0.55	10.97	5.78	31.87	114.50	631.06	10.44	57.55	64.38	61.97	5.51	5.30
Mono Q	3.81	0.81	3.10	10.03	50.00	38.23	190.50	12.33	61.48	21.49	18.71	6.50	5.66

^aDirect oxidation or Mn²⁺-mediated oxidation was calculated from the oxidation values measured in the absence or presence of Mn²⁺ in the reaction mixtures (after excluding the activity in the absence of H₂O₂), respectively.

^bEnzyme activity values are based on direct oxidation (left) or Mn²⁺-mediated oxidation (right) of phenol red.

^cResidual fraction.