TABLE 3.
Kinetic constants of P. ostreatus VP1
| Testing substrate | pH | Additional substrate | Km (μM) | Vmax (μmol/s) | Kcat (1/s) | Kcat/Km (1/s · mM) |
|---|---|---|---|---|---|---|
| H2O2a | 5 | 2 ± 10 | 0.03 ± 14.5 | 0.6 ± 290 | 5,961 ± 29,215 | |
| Mn2+ | 5 | 171 ± 9.6 | 0.3 ± 8.8 | 6.2 ± 176 | 80.3 ± 1,038 | |
| Mn2+ | 5 | 25 μM OII | 156 ± 1.7 | 0.2 ± 8.36 | 3.5 ± 167.2 | 28.3 ± 1,068 |
| Mn2+ | 5 | 50 μM OII, pH 5 | Complete inhibition | |||
| Mn2+ | 5 | 25 μM RB5 | 155 ± 8.1 | 7 ± 83.7 | 140 ± 1,674.4 | 1,485 ± 10,862 |
| Mn2+ | 5 | 50 μM RB5 | 55 ± 11.7 | 1.6 ± 8.3 | 31.6 ± 165.5 | 100 ± 2,746 |
| OII | 3 | 35.8 ± 1.8 | 0.04 ± 0.26 | 0.83 ± 5.1 | 15.7 ± 142.7 | |
| OII | 3 | 50 μM Mn2 | 58.7 ± 10 | 0.37 ± 0.05 | 7.3 ± 0.94 | 126.4 ± 12.4 |
| OII | 3 | 500 μM Mn2+ | Complete inhibition | |||
| OII | 3 | 25 μM RB5 | 210.7 ± 24 | 2 ± 0.14 | 40 ± 2.7 | 26.6 ± 202 |
| RB5 | 3 | 24.8 ± 5.2 | 0.54 ± 0.04 | 54 ± 3.5 | 2,224.4 ± 365 | |
| RB5 | 3 | 50 μM Mn2+ | 40.2 ± 7.8 | 0.65 ± 0.12 | 65.4 ± 12 | 1,631 ± 40 |
| RB5 | 3 | 500 μM Mn2 | Complete inhibition | |||
| RB5 | 3 | 25 μM OII | Complete inhibition |
a
In this reaction, Mn2+ was oxidized at a different concentration of H2O2.