TABLE 1.
COMSTAT analysis of biofilms formed by mutants lacking major biofilm regulatorsa
| Strain/genotype | Temp (°C) | Biomass (μm3/μm2) | Thickness (μm) |
Roughness coefficient | |
|---|---|---|---|---|---|
| Avg | Maximum | ||||
| WT | 37 | 0.22 (0.02) | 0.30 (0.09) | 5.57 (1.02) | 1.83 (0.04) |
| 25 | 1.64 (0.25) | 1.89 (0.15) | 15.55 (1.02) | 1.19 (0.05) | |
| 15 | 6.64 (0.23) | 7.33 (0.24) | 22.00 (2.33) | 0.43 (0.02) | |
| ΔaphA | 37 | 0.12 (0.02) | 0.13 (0.02) | 7.63 (4.06) | 1.92 (0.02) |
| 25 | 2.71 (0.28) | 3.35 (0.12) | 18.77 (3.09) | 0.78 (0.06) | |
| 15 | 7.41 (0.37) | 7.73 (0.42) | 23.17 (1.02) | 0.40 (0.01) | |
| ΔvpsR | 37 | 0.17 (0.03) | 0.19 (0.02) | 5.87 (0.51) | 1.89 (0.02) |
| 25 | 1.17 (0.18) | 1.22 (0.07) | 6.45 (1.02) | 1.30 (0.05) | |
| 15 | 3.25 (0.28) | 3.09 (0.31) | 6.16 (0.88) | 0.30 (0.05) | |
| ΔvpsT | 37 | 0.16 (0.01) | 0.17 (0.03) | 5.28 (0.88) | 1.89 (0.01) |
| 25 | 0.87 (0.47) | 1.17 (0.47) | 7.33 (0.51) | 1.40 (0.25) | |
| 15 | 2.37 (0.36) | 2.62 (0.27) | 9.68 (0.88) | 0.54 (0.06) | |
a
Biofilms were grown at 15°C, 25°C, and 37°C in static chambers in LB medium for 24 h. Values in parentheses are standard deviations.