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. 2016 Jun 30;82(14):4350–4362. doi: 10.1128/AEM.00906-16

FIG 3.

FIG 3

Gel mobility shift assay of AtzR and NtrC on the PatzT promoter region. Autoradiograph of a representative PAGE gel. Lane 1 shows a free PatzT probe. Lanes 2 to 6 show a preformed complex containing 2 μM NtrCD55E,S161F challenged with 0 nM (lane 2), 50 nM (lane 3), 100 nM (lane 4), 200 nM (lane 5), or 400 nM AtzR-His6. Lanes 7 to 11 show a preformed complex containing 400 nM AtzR-His6, challenged with 0 nM (lane 7), 250 nM (lane 8), 500 nM (lane 9), 1 μM (lane 10), or 2 μM (lane 11) NtrCD55E,S161F. AtzR-DNA, NtrC-DNA, and AtzR-NtrC-DNA complexes are denoted by black, gray, and white arrows, respectively.