FIG 5.

Fatty acid supplementation shows variable protection in daptomycin-sensitive (S613) and daptomycin-resistant (R712) clinical pair isolates versus high bile challenge. Shown are the averages ± standard deviations for n = 3. (A) Bile supplementation and challenge with 20% bovine bile. All strains supplemented with 0.2% bile had statistically increased numbers of survivors versus those of their unsupplemented counterparts at all analyzed time points (P ≤ 0.001). (B) Oleic acid (OA) supplementation and challenge with 20% bovine bile. All strains supplemented with 20 μg/ml OA versus those of their unsupplemented counterparts were not statistically different at all time points analyzed (P value > 0.05).