TABLE 1.
Strain | Generation times in medium constituent (min) |
||||
---|---|---|---|---|---|
BHIa | Serumb | Bilec | C18:1 cis9d | Ethanole | |
WTf | 32.2 ± 0.5 | 36.5 ± 0.8 | 32.7 ± 1.9 | 39.6 ± 0.7 | 42.1 ± 1.0 |
ΔliaR | 28.7 ± 1.3 | 36.2 ± 3.4 | 33.2 ± 3.4 | 35.2 ± 3.3 | 38.0 ± 4.1 |
ΔliaR::liaR | 31.4 ± 2.9 | 34.1 ± 2.8 | 27.5 ± 3.4 | 35.2 ± 2.4 | 35.0 ± 1.9 |
S613 | 29.7 ± 2.7 | 31.0 ± 3.4 | 48.1 ± 3.5 | 27.1 ± 1.5 | 31.1 ± 2.6 |
R712 | 29.4 ± 2.5 | 36.0 ± 1.2 | 40.8 ± 3.8 | 30.0 ± 2.2 | 38.0 ± 1.0 |
BHI medium was used in all cultures with supplements as indicated.
Pooled human serum was supplemented to a final concentration of 15%.
Bovine bile was supplemented to a final concentration of 0.2%.
Oleic acid was added to a final concentration of 20 μg/ml.
Ethanol solvent control was added to a final concentration of 0.2%.
WT, wild type.