TABLE 2.
Membrane analysis of wild-type and mutant strains during log phase growth
| Fatty acid | % of total membrane content for indicated supplement and strain (avg ± SD)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BHI |
Serumb |
Bilec |
C18:1
cis9d |
|||||||||
| WTe | ΔliaR | ΔliaR::liaR | WT | ΔliaR | ΔliaR::liaR | WT | ΔliaR | ΔliaR::liaR | WT | ΔliaR | ΔliaR::liaR | |
| C12:0 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.9 ± 0.1 | ND | 0.3 ± 0.0 | 0.3 ± 0.0 | ND | ND | 0.2 ± 0.2 | 0.7 ± 0.1 | 1.1 ± 0.3 | 1.1 ± 0.1 |
| C14:0 | 4.6 ± 0.1 | 4.4 ± 0.0 | 6.4 ± 0.1 | 2.4 ± 0.1 | 2.6 ± 0.1 | 2.9 ± 0.1 | 1.1 ± 0.0 | 1.0 ± 0.0 | 1.6 ± 0.1 | 0.7 ± 0.1 | 0.9 ± 0.3 | 0.5 ± 0.1 |
| C16:1 | 7.0 ± 0.1 | 6.5 ± 0.4 | 9.7 ± 0.1 | 4.9 ± 0.1 | 4.9 ± 0.1 | 5.1 ± 0.1 | 1.5 ± 0.5 | 1.5 ± 0.0 | 1.7 ± 0.1 | 1.8 ± 0.2 | 1.6 ± 0.1 | 1.2 ± 0.1 |
| C16:0 | 37.6 ± 0.6 | 37.4 ± 0.2 | 34.9 ± 0.3 | 35.9 ± 0.6 | 36.1 ± 0.4 | 37.2 ± 0.3 | 42.6 ± 0.1 | 42.6 ± 0.2 | 42.4 ± 0.2 | 3.4 ± 0.5 | 3.1 ± 0.7 | 1.7 ± 0.3 |
| C17:1 | ND | 1.5 ± 0.1 | ND | 0.6 ± 0.0 | 0.6 ± 0.0 | 0.5 ± 0.0 | 1.7 ± 0.2 | 1.0 ± 0.0 | 1.7 ± 0.1 | 0.3 ± 0.3 | 0.4 ± 0.3 | 0.7 ± 0.2 |
| C17:0 2OH | 5.2 ± 0.7 | 5.2 ± 0.8 | 5.1 ± 0.4 | 0.2 ± 0.3 | 0.6 ± 0.1 | 0.5 ± 0.0 | 0.3 ± 0.2 | 0.3 ± 0.0 | 0.3 ± 0.0 | ND | ND | ND |
| C18:1 cis9 | 0.7 ± 0.7 | 0.2 ± 0.4 | ND | 21.2 ± 0.3 | 20.3 ± 0.9 | 18.4 ± 0.2 | 42.3 ± 0.2 | 38.4 ± 1.8 | 40.8 ± 0.5 | 76.3 ± 1.0 | 68.5 ± 5.0 | 75.1 ± 0.7 |
| C18:1 cis11 | 39.0 ± 0.5 | 38.5 ± 1.4 | 38.9 ± 0.5 | 4.7 ± 0.1 | 4.9 ± 0.2 | 4.4 ± 0.2 | 3.7 ± 0.1 | 3.8 ± 0.1 | 3.6 ± 0.0 | ND | ND | ND |
| C18:0 | 4.7 ± 0.1 | 5.0 ± 0.2 | 2.9 ± 0.1 | 9.2 ± 0.2 | 9.1 ± 0.1 | 7.8 ± 0.1 | 2.8 ± 0.1 | 6.9 ± 0.8 | 3.0 ± 0.3 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.5 ± 0.1 |
| C20:0 | ND | ND | ND | ND | ND | ND | 0.6 ± 0.1 | 0.5 ± 0.0 | 0.4 ± 0.1 | 15.9 ± 0.7 | 23.7 ± 4.1 | 19.2 ± 0.9 |
| C18:2 | ND | ND | ND | 18.5 ± 0.5 | 18.1 ± 0.3 | 20.7 ± 0.2 | ND | ND | ND | ND | ND | ND |
| Othersf | 0.2 ± 0.4 | 0.3 ± 0.4 | 1.2 ± 1.1 | 2.5 ± 0.4 | 2.5 ± 1.6 | 2.3 ± 0.5 | 3.5 ± 0.3 | 3.9 ± 0.9 | 4.3 ± 0.8 | ND | ND | ND |
| Saturated/unsaturated | 1.0 ± 0.6 | 1.0 ± 0.2 | 0.9 ± 0.5 | 0.9 ± 0.9 | 1.0 ± 0.4 | 1.0 ± 0.7 | 0.9 ± 0.5 | 1.1 ± 0.5 | 1.0 ± 1.1 | 0.3 ± 1.0 | 0.4 ± 1.0 | 0.3 ± 1.4 |
| C10-C17/C18-C20g | 1.2 ± 0.03 | 1.3 ± 0.4 | 1.4 ± 0.03 | 0.8 ± 0.02 | 0.9 ± 0.03 | 0.9 ± 0.01 | 1.0 ± 0.005 | 1.0 ± 0.02 | 1.0 ± 0.01 | 0.1 ± 0.01 | 0.1 ± 0.01 | 0.1 ± 0.01 |
a
Membrane contents were determined by GC-FAME analysis by Microbial ID, Inc. Values represent averages and SD from for three independent cultures. ND indicates that fatty acid was not detected.
b
Pooled human serum was supplemented to a final concentration of 15%.
c
Bovine bile was supplemented to a final concentration of 0.2%.
d
Oleic acid was added to a final concentration of 20 μg/ml.
e
WT, wild type.
f
Others indicates fatty acids that comprised <1% of the total membrane content.
g
Total fatty acid length ratios including both saturated and unsaturated fatty acids.