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. 2016 Jun 30;82(14):4264–4278. doi: 10.1128/AEM.00463-16

FIG 4.

FIG 4

Lipid fractions from SPF and GF mouse feces and intestinal mucosa display similar major mass ion species that correspond to cardiolipin isoforms. Negative ion MALDI-TOF MS was performed to assess the predominant mass ions present in chloroform-methanol lipid extracts of the feces and intestinal mucosa of SPF and GF mice. The atomic mass units assigned to the major peaks are indicated. (A to D) Major negative mass ions present in SPFfeccm (A), GFfeccm (B), SPFimcm (C), and GFimcm (D). (E) Structure of the major cardiolipin [1,3-bis(3-sn-phosphatidyl)glycerol] species corresponding to the negative mass ions present in GFfeccm, SPFfeccm, GFimcm and SPFimcm. R1 to R4, fatty acids residues attached to the side chain glycerol residues. The positions of the 1H NMR signals for protons of side chain glycerol residues CH2OR1(4), CHOR2(3), and CH2OP(O) and for protons of the central glycerol CH2OP(O) and CHOH are shown in Table 1. The side chain glycerol residues are indicated by dotted lines. intens., intensity; a.u., atomic mass units.