HS induced the upstream expression of HSPs. The wild-type strains were cultured on PDA plates for 5 days and then exposed to 42°C for 0 min to 60 min. The expression levels of the hsp17.4 (A), hsp22 (B), hsp70 (C), and hsp90 (D) genes were measured immediately after HS. The expression level of the hsp17.4, hsp22, hsp70, or hsp90 gene from the wild-type strains without heat stress treatment was arbitrarily designated a value of 1. The values are the means ± SD of the results from three independent experiments. Asterisks indicate significant differences from untreated strains (**, P < 0.01, Student's t test).