FIG 6.

Effect of Ca²⁺ inhibitors on the expression of HSPs in heat-stressed strains. The wild-type strains were cultured on PDA for 5 days and then treated with 1 mM BAPTA, 5 mM EGTA, 10 mM LaCl₃, or 1 mM neomycin for 30 min. The strains were exposed to 42°C for 20 min. The expression levels of the hsp17.4 (A), hsp22 (B), hsp70 (C), and hsp90 (D) genes were measured immediately after HS. The values are the means ± SD of the results from three independent experiments. Asterisks indicate significant differences from the heat-stressed-only strains (*, P < 0.05; **, P < 0.01, Student's t test).