TABLE 2.
Results of fermentation processes on the 2-liter scale after strain improvement by metabolic engineering
| Strain | Total amount of ferulic acid supplied (g) | Amount of ferulic acid catabolized (g) | Final concentration of vanillin (g/liter) | Total amount of vanillin produced (g) | Molar yielda (%) |
|---|---|---|---|---|---|
| F84 | 49.5 | 48.7 ± 0.2 | 19.3 ± 0.2 | 36.8 ± 0.2 | 94.9 ± 0.4 |
| F33 | 38.5 | 35.5 ± 0.6 | 14.4 ± 1 | 24.4 ± 1.4 | 80.9 ± 4.6 |
| ATCC 39116 | 38.5 | 35.7 ± 0.8 | 12.2 ± 2.3 | 20.7 ± 3.1 | 68.6 ± 10.4 |
a
Yield of ferulic acid to vanillin.