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Colony immunostaining with anti-β-(1→6)-glucan serum of different M. agalactiae 5632 transformants. Different OriC/Tetr-encoding plasmids, containing (i) no gene (pO/T), (ii) the native MAGb_1260 gene (pO/T1260), or (iii) a modified MAGb_1260 gene with no poly(G) tract (pO/T1260mod) under the control of the P40 promoter, were used for transformation. The population consisted of colonies that were positive (P; dark blue), negative (N; pink), or sectored (S; red arrow).
