TABLE 4.
Length of the poly(G), 3′ sequence tract in gsmA and β-(1→6)-glucan secretion in M. agalactiae field isolates
| Strain | Origin | Host | Clinical sign | Yr | Poly(G) and 3′ sequencea | Length of the poly(G) sequence (nt) | gsmA in frame | β-(1→6)-Glucan secretionb |
|---|---|---|---|---|---|---|---|---|
| 15291 | France | Goat | Arthritis | 1987 | GGGGGGGGGGAC TTA TTC | 10 | Yes | Yes |
| 14628 | France | Ibex | Pneumonia | 2006 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 15341 | France | Chamois | Pneumonia | 2009 | GGGGGGGGG AAA CTA ATC | 9 | Yes | Yes |
| 13387 | France | Ibex | Pneumonia | 2003 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 15310 | France | Ibex | Pneumonia | 2009 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 7323 | Greece | Goat | Mastitis | 1993 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 7320 | Greece | Sheep | Mastitis | 1993 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 7337 | Greece | Goat | Mastitis | 1993 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| 13378 | France | Goat | None | 2003 | GGGGGGGGG AAC TTA TTC | 9 | Yes | Yes |
| PG2T | Spain | Goat | Unknown | 1952 | GGGGGGGG- AAC TTA TTC | 8 | No | No |
| 4908 | France | Goat | Mastitis | 1990 | GGGGGGGAC --- TTA TTC | 7 | Yes | Yes |
| 6355 | Spain | Sheep | Arthritis | 1992 | GGGGGGGAC --- TTA TTC | 7 | Yes | Yes |
| 5632 | Spain | Goat | Arthritis | <1991 | GGGGGC --- AAC TTG TTC | 5 | Noc | No |
| 14668 | France | Goat | Pneumonia | 2006 | GGGGGC --- AAC TTG TTC | 5 | Yes | Yes |
| 4867 | Spain | Goat | Mastitis | 1990 | GGGGGC --- AAC TTG TTC | 5 | Noc | No |
a
Sequence details of the gsmA locus containing the poly(G) stretch (indicated with underlined boldface characters). Nucleotides are grouped in codons according to the open reading frame. Base deletions are indicated by hyphens.
b
As estimated by the majority of colonies positively stained by mmc4 antiserum.
c
gsmA is disrupted by an insertion sequence (ISmag1) or by a single base deletion upstream of the poly(G) sequence for strain 5632 or strain 4867, respectively.