TABLE 3.
RT-PCR gene expression in four Mycobacterium strains grown with single (PYR and PHE) and mixed (MIX) substrates at 3/7 days of incubation
| Gene | Substrate treatment by bacterial straina |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| czh-101 |
JS19b1 |
czh-3 |
czh-117 |
|||||||||
| PYR | MIX | PHE | PYR | MIX | PHE | PYR | MIX | PHE | PYR | MIX | PHE | |
| nidA | +/+ | +/+ | +/+ | −/− | −/− | −/− | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| nidB | +/+ | +/+ | +/+ | −/− | −/− | −/− | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| phdF | +/+ | +/+ | +/+ | −/− | +/− | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| phdI | +/+ | +/+ | +/+ | −/− | −/− | −/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| phdJ | +/+ | +/+ | +/+ | NA | NA | NA | +/+ | +/+ | +/+ | −/+ | +/+ | +/+ |
| phtAa | +/+ | +/+ | +/− | −/− | −/− | +/+ | +/+ | +/+ | +/+ | −/− | −/− | −/− |
a
+, gene amplified; −, gene not amplified; NA, gene not found in the genome of the strain. For all strains and treatments, the main housekeeping sigma factor (sigA) was amplified from cDNA as a positive control for amplification.