TABLE 2.
Estimated genome sizes of Blissus insularis-associated symbiont Burkholderia isolates detected by PFGE and of Burkholderia reference strains
| Cladea | Strain designation | No. of replicatesb | Mean (SE) size (Mb)c of: |
Total (SE) size (Mb) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Replicon 1 | Replicon 2 | Replicon 3 | Replicon 4 | Replicon 5 | Replicon 6 | ||||
| SBE | Bi16MC_R_vitro | 2 | 3.4 (0) | 1.8 (0) | 1.6 (0) | 0.5 (0) | 7.3 (0) | ||
| Bi18MC_R_vitro | 2 | 3.0 (0.1) | 1.3 (0.1) | 1.2 (0) | 1.1 (0) | 0.5 (0) | 0.5 (0) | 7.5 (0.1) | |
| Bi22MC_R_vitro | 2 | 3.1 (0) | 1.5 (0) | 1.2 (0) | 1.0 (0) | 0.5 (0) | 0.5 (0) | 7.7 (0) | |
| Bi23MC_R_vitro | 2 | 3.0 (0) | 1.4 (0) | 1.0 (0) | 0.8 (0) | 0.5 (0) | 6.8 (0) | ||
| Bi12MC_S_vitro | 2 | 3.0 (0.1) | 1.4 (0.1) | 1.0 (0) | 0.7 (0) | 0.6 (0) | 6.7 (0.2) | ||
| Bi14MC_S_vitro | 2 | 3.1 (0) | 1.4 (0) | 1.0 (0) | 0.8 (0) | 0.6 (0) | 6.8 (0) | ||
| Burkholderia sp. strain RPE67 | 8.7d | ||||||||
| Burkholderia sp. strain YI23 | 8.9e | ||||||||
| PBE | Bi20MC_R_vitro | 3 | 3.5 (0) | 2.0 (0) | 1.3 (0) | 1.2 (0) | 8.0 (0) | ||
| Bi21MC_R_vitro | 5 | N/A | N/A | ||||||
| Bi16MC_S_vitro | 2 | N/A | N/A | ||||||
| Bi19MC_S_vitro | 3 | N/A | N/A | ||||||
| Burkholderia caribensis | 9.0f | ||||||||
| BCC | Bi17MC_R_vitro | 2 | 3.4 (0) | 2.8 (0) | 1.0 (0) | 7.2 (0) | |||
| Bi19MC_R_vitro | 3 | N/A | N/A | ||||||
| Bi24MC_R_vitro | 2 | 4.4 (0) | 3.6 (0.1) | 0.7 (0) | 8.7 (0.1) | ||||
| Bi25MC_R_vitro | 2 | 4.5 (0) | 3.8 (0) | 8.3 (0) | |||||
| Bi20MC_S_vitro | 2 | 3.7 (0.1) | 2.3 (0) | 0.7 (0) | 6.6 (0.1) | ||||
| Bi21MC_S_vitro | 2 | 4.4 (0.1) | 3.8 (0) | 8.2 (0.1) | |||||
| Bi22MC_S_vitro | 2 | 4.4 (0) | 3.7 (0) | 8.1 (0) | |||||
| Bi26MC_S_vitro | 2 | 4.4 (0) | 3.7 (0) | 8.1 (0) | |||||
| Bi27MC_S_vitro | 3 | 3.6 (0) | 2.6 (0) | 0.9 (0) | 0.4 (0) | 0.4 (0) | 7.9 (0) | ||
| Bi28MC_S_vitro | 2 | 3.6 (0) | 2.6 (0.1) | 1.0 (0) | 7.2 (0.1) | ||||
| Burkholderia gladioli | 9.1g | ||||||||
| Burkholderia cepacia | 6.4h | ||||||||
Defined by the universal 16S rRNA gene sequences (see Fig. 3 for details).
Number of replicates conducted using freshly prepared sample plugs on separate PFGE gels.
Replicons were separated on a 0.8% pulsed-field-certified agarose without restriction digestion. N/A, not available due to DNA degradation (a smear pattern) during PFGE (see Fig. S5 in the supplemental material).
Burkholderia sp. strain RPE67 has three chromosomes (3.1, 1.8, and 1.7 Mb) and three plasmids (1.4, 0.5, and 0.2 Mb). See reference 61.
Burkholderia sp. strain YI23 has three chromosomes (3.1, 1.8, and 1.6 Mb) and three plasmids (1.9, 0.4, and 0.1 Mb). See reference 65.
Burkholderia caribensis has two chromosomes (3.7 and 2.9 Mb) and two plasmids (2.0 and 0.4 Mb). See reference 66.
Burkholderia gladioli has two chromosomes (4.4 and 3.7 Mb) and four plasmids (0.4, 0.3, 0.1, and 0.1 Mb). See reference 67.
Data from the draft genome sequence of Burkholderia cepacia (68).