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. 2016 May 16;82(11):3289–3296. doi: 10.1128/AEM.03931-15

TABLE 1.

Oligonucleotide probes used in this studya

Probe name Target group Probe sequence (5′→3′) Length (nt)b FA (%)c Reference
CF319a Bacteroidetes TGGTCCGTGTCTCAGTAC 18 35 29
SAR11-152R SAR11 clade ATTAGCACAAGTTTCCYCGTGT 22 25 30
SAR11-441R SAR11 clade TACAGTCATTTTCTTCCCCGAC 22 25 30
SAR11-441R(modif) SAR11 clade TACCGTCATTTTCTTCCCCGAC 22 25 31
SAR11-487(modif) SAR11 clade CGGACCTTCTTATTCGGG 18 25 31
SAR11-487-H3d SAR11 clade CGGCTGCTGGCACGAAGTTAGC 22 25 31
SAR11-542R SAR11 clade TCCGAACTACGCTAGGTC 18 25 30
SAR11-732R SAR11 clade GTCAGTAATGATCCAGAAAGYTG 23 25 30
EUB338 I Bacteria GCTGCCTCCCGTAGGAGT 18 35 32
EUB338 II Supplement to EUB338 GCAGCCACCCGTAGGTGT 18 35 33
EUB338 III Supplement to EUB338 GCTGCCACCCGTAGGTGT 18 35 33
a

All Bacteria-specific probes (EUB338 I, EUB338 II, EUB338 III) were applied together as a mix. This applied also to all SAR11-specific probes.

b

nt, nucleotides.

c

FA, formamide concentration (vol/vol) in the hybridization buffer.

d

Unlabeled helper oligonucleotide to probe SAR11-487(modif).