TABLE 1.
Summary of groundwater sample information in this studya
| Location (abbreviation) | Well ID no. | Sample designation | Sampling date (mo/day/yr) | DNA concn (ng/μl) | VC concn (μg/liter) | Ethene concn (μg/liter) | DO concn (mg/liter) | kpoint (yr−1)b | kbulk (yr−1)b | EaCoMT gene abundance (genes/liter)c | Diversity (H′ and 1/D) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Carver, MA (CARV) |
RB46D | CARV46 | 9/15/2010 | 3.25 | 1.5 | NA | 0.65 | 0.365 (P < 0.001) | 0.159 (P = 0.001) | 1.2 × 105 | NA |
| RB63I | CARV63 | 9/15/2010 | 3.95 | 1.3 | NA | 0.29 | 0.183 (P < 0.001) | 0.159 (P = 0.001) | 1.9 × 104 | 0.73 ± 0.01 1.55 ± 0.02 | |
| RB46D | CARV46-1 | 9/29/2009 | NA | 1.8 | 3.9 | 0.31 | 0.365 (P < 0.001) | 0.119 (P = 0.001) | NA | NA | |
| RB64I | CARV64 | 9/29/2009 | NA | <0.46 | <0.1 | 0.45 | 0.1095 (P < 0.001) | 0.119 (P = 0.001) | NA | NA | |
| Soldotna, AK (SOLD) | MW6 | SOLD6 | 05/12/2009 | 1.00 | 9.6 | 50 | 0.53 | 0.621 (P < 0.001) | 0.992 (P = 0.050) | 6.3 × 105 | 1.05 ± 0.50 2.28 ± 0.94 |
| MW40 | SOLD40 | 09/22/2008 | 2.61 | 20.7 | 90 | 1.29 | 0.730 (P < 0.001) | 2.093 (P = 0.059) | 1.6 × 105 | 1.53 ± 0.15 2.64 ± 0.38 | |
| Oceana, VA (OCEA) | MW18 | OCEA18 | 08/06/2009 | 0.14 | 0.8 | <1 | 1.89 | 0.730 (P < 0.001) | 0.008 (P = 0.524) | 4.3 × 103 | 1.29 ± 0.22 3.48 ± 0.64 |
| MW25 | OCEA25 | 11/21/2008 | 8.96 | 19 | <1 | 1.20 | 0.256 (P = 0.132) | 0.003 (P = 0.379) | 2.4 × 104 | 1.54 ± 0.14 3.73 ± 0.38 | |
| Melbourne, Australia (AUS) | 039IJ-1 | AUS39-1 | 10/10/2011 | 1.24 | 72,000 | 780 | 0.07 | NA | 0.368 (P = 0.034) | 8.6 × 106 | 1.47 ± 0.00 3.93 ± 0.01 |
| 039IJ-3 | AUS39-3 | 10/10/2011 | 0.32 | 4,400 | NA | 0.28 | NA | 0.368 (P = 0.034) | 4.7 × 104 | 0.98 ± 0.07 2.12 ± 0.04 | |
| 039IJ-6 | AUS39-6 | 10/17/2011 | 1.00 | 53,000 | 230 | 1.07 | NA | 0.368 (P = 0.034) | 3.0 × 106 | 1.17 ± 0.03 2.45 ± 0.04 | |
| 039IJ-7 | AUS39-7 | 10/17/2011 | 0.78 | 15,000 | 40 | 2.2 | NA | 0.368 (P = 0.034) | 3.0 × 105 | 1.09 ± 0.03 2.41 ± 0.05 | |
| 039IJ-8 | AUS39-8 | 10/18/2011 | 2.28 | 24,000 | NA | 0.65 | NA | 0.368 (P = 0.034) | 1.3 × 106 | 1.08 ± 0.07 2.36 ± 0.08 | |
| Kotzebue, AK (KOTZ) | MW10-01 | KOTZ01 | 10/22/2013 | 10.4 | <0.62 | NA | 1.46 | NA | NA | 6.6 × 105 | 0.95 ± 0.09 2.11 ± 0.33 |
| MW10-03 | KOTZ03 | 10/22/2013 | 1.31 | <0.62 | NA | 8.70 | NA | NA | 9.8 × 104 | 0.91 ± 0.08 2.15 ± 0.05 | |
| Fairbanks, AK (FAIR) | MW-4 M | FAIR4 | 03/27/2014 | 15.7 | <0.4 | <0.06 | 1.36 | NA | NA | 7.0 × 103 | 1.39 ± 0.42 2.87 ± 1.13 |
| MW-13 M | FAIR13 | 03/27/2014 | 14.3 | <0.4 | <0.06 | 0.88 | NA | NA | 3.9 × 104 | 1.68 ± 0.15 3.54 ± 0.45 |
VC, ethene, and geochemical data were provided by personal communication and/or publicly available reports as follows: CARV, James Begley of MT Environmental Restoration and Sam Fogel of Bioremediation Consulting, Inc.; SOLD, May 2009 Groundwater Monitoring Report, River Terrace RV Park, Soldotna, AK, Alaska Department of Environmental Conservation via Tim McDougall of Oasis Environmental and James Fish of Alaska Department of Environmental Quality; OCEA, Long-term Monitoring Report (2009) for SMWUs 2B, 2C, and 2E, Oceana NAS, Virginia Beach, VA, via Laura Cook of CH2MHill; AUS, Dora Ogles from Microbial Insights, Inc.; KOTZ and FAIR, James Fish of Alaska Department of Environmental Conservation. NA, not analyzed.
kpoint and kbulk are estimated values of the point VC decay rate and bulk VC attenuation rate, respectively. Statistically significant k values are based on P values of <0.1.
Quantification of etnE genes at CARV, SOLD, and OCEA were published previously (36) and are provided here for reference and used in correlation analyses.