TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description or relevant genotypea | Source or reference |
|---|---|---|
| Strains | ||
| Burkholderia plantarii | ||
| MAFF301723 | Wild type | NIASb |
| KE1 | MAFF301723 troR1::pK18mobsacB | This study |
| KE2 | MAFF301723 troK::pK18mobsacB | This study |
| KE3 | MAFF301723 troR2::pK18mobsacB | This study |
| Escherichia coli DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 λ− | Thermo |
| Plasmids | ||
| pK18mobsacB | oriT oriV sacB lacZα Kmr | 20 |
| pK18-HK1-55c | 60% from ATG initiation codon of full length of 55 HK genes cloned into HindIII site of pk18mobsacB | This study |
| pK18-RR1-72c | 60% from ATG initiation codon of full length of 72 RR genes cloned into HindIII site of pk18mobsacB | This study |
| pMLBAD | pBRR1 ori araC-pBAD Tpr mob+ | 21 |
| pMLBAD-troR1+ | troR1+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troK+ | troK+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troR2+ | troR2+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troR1+-K+ | troR1+ and troK+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troK+-R2+ | troK+ and troR2+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troR1+-K+-R2+ | troR1+, troK+, and troR2+ cloned between NcoI and XbaI sites of pMLBAD | This study |
| pMLBAD-troR1D47A-K+-R2+ | pMLBAD-troR1-K+-R2+ troR1D47A | This study |
| pMLBAD-troR1D52A-K+-R2+ | pMLBAD-troR1-K+-R2+ troR1D52A | This study |
| pMLBAD-troKH253A-R2+ | pMLBAD-troK-R2+ troKH253A | This study |
| pMLBAD-troKH443A-R2+ | pMLBAD-troK-R2+ troKH443A | This study |
| pMLBAD-troR2D32A | pMLBAD-troR2 troR2D32A | This study |
| pMLBAD-troR2D46A | pMLBAD-troR2 troR2D46A | This study |
a
Kmr, kanamycin resistant; Tpr, tropolone resistant. troR1D47A, troR1D52A, troKH253A, troKH443A, troR2D32A, and troR2D46A, troR1, troK, and troR2 genes encoding substitutions D47A, D52A, H253A, H443A, D32A, and D46A, respectively.
b
NIAS, National Institute of Agrobiological Science.
c
Fifty-five HKs and 75 RRs were identified by analysis of MAFF301723 whole-genome draft sequence data; 72 of 75 RR genes were cloned.