TABLE 2.
Complementation assaya
| Strain | Tropolone production after transformation with plasmid: |
|||||
|---|---|---|---|---|---|---|
| pMLBAD-troR1+ | pMLBAD-troK+ | pMLBAD-troR2+ | pMLBAD-troR1+-K+ | pMLBAD-troK+-R2+ | pMLBAD-troR1+-K+-R2+ | |
| KE1 | − | − | − | − | − | + |
| KE2 | − | − | − | − | + | + |
| KE3 | − | − | + | − | + | + |
a
Plasmids pMLBAD-troR1+, pMLBAD-troK+, pMLBAD-troR2+, pMLBAD-troR1+-K+, pMLBAD-troK+-R2+, and pMLBAD-troR1+-K+-R2+ were each independently transformed into KE1, KE2, and KE3. These transformants were cultured in PY medium overnight. They were spread on modified Hoitink agar (100 μg/ml kanamycin, 50 μg/ml tropolone, 0.02% arabinose) and incubated at 30°C. Tropolone-producing colonies appeared as red-brown (+), and tropolone-nonproducing colonies appeared as white (−). The tropolone production of the strains was also confirmed by the measurement of absorbance at 330 nm.