FIG 3.

ZapD-mediated FtsZ sedimentation assays. (A) Representative Coomassie-stained gels showing the sedimentation profiles of FtsZ with WT ZapD and FtsZ with ZapD R225A. All reactions were prepared with 4.8 μM FtsZ, and ZapD was added at the concentrations indicated. Fractions analyzed included the following: I, the initial protein input to each reaction; S, the soluble portion after centrifugation; and P, the pellet portion. The lane designated -ve control represents bundling assay with 4.8 μM FtsZ and ZapD in the absence of GTP. (B) Sedimentation profiles were assessed by densitometry for WT ZapD and all ZapD variants in triplicate (±standard errors). The densitometric data were plotted as the percentage of FtsZ sedimented, relative to the initial input for each reaction, versus the concentration of ZapD.