TABLE 4.
Summary of results from FtsZ bundling and sedimentation by ZapD variants
| ZapD variant | EC50a (μM) | Max sed.b (%) | No. of filaments/bundlec |
|---|---|---|---|
| WT | 1.86 | 79.5 | 12 |
| R16A variant | 1.26 | 96.5 | 11 |
| R20A variant | 2.49 | 73.6 | 12 |
| R56A variant | 5.92 | 53.9 | 7** |
| E114A variant | 1.74 | 77.9 | 16* |
| R116A variant | 2.77 | 73.8 | NA |
| R124A variant | 1.93 | 92.6 | 11 |
| R176A variant | 2.64 | 80.6 | 11 |
| R221A variant | 3.19 | 72.1 | NA |
| R225A variant | 9.08 | 49.2 | NA |
a
EC50 corresponds to the concentration of ZapD required to sediment 50% of FtsZ in solution.
b
Max sed. is the maximum observed sedimentation of FtsZ in the triplicate FtsZ sedimentation assays.
c
The number of filaments per bundle was counted for each sample from TEM images (n = 50). **, P value of <0.0001 compared to WT results; *, P value of <0.05 compared to WT results. NA, not applicable.