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Binding of Stx B subunits in real time as determined by BLI. Gb3-PC-Ch was immobilized on a hydrophobic sensor tip. Association was assessed in wells containing various concentrations of B subunits of Stx1 (A), Stx2a WT (B), and Stx2a Q40L (C) for 900 s, and then the tips were transferred to wells containing only buffer and dissociation was monitored. Means for three independent repeats were plotted. (D) Equilibrium binding at 900 s of association.
